The triple-negative breast cancer MDA-MB-231 cells expressing both EGFR and PD-L1 (Figure 2d) were co-cultured for 48 h at two different effector/target ratios (1:1 and 2:1) with anti-CD3-prestimulated PBMCs expressing PD-1 and 4-1BB (Figure 2e). This evidence concerns the gene PDCD1 and triple-negative breast carcinoma.