Ephrin type-B receptor 4 (EPHB4) has been responsible and expressed in tumours which makes a good target for CAR-T cells. PB transposon was used to generate EPHB4-CAR-T cells which allowed for sufficient T cell proliferation. These cells able to stop positive tumour cells expressing EPHB4 without activating cell proliferation. The gene discussed is EPHB4; the disease is neoplasm.