CRISPR/Cas9 was used to create an HSV-based virus, where coding region ICP34.5 was replaced by therapeutic genes murine (IL12, p40-p35) and C-X-C motif chemokine ligand 11, and the deletion of ICP47 gene. The combination of IL12 and CXCL11 with oncolytic virus have a promising treatment in colon cancer. Here, CXCL11 is linked to malignant colon neoplasm.