Furthermore, knock-out of MFGE8 in CRC-EVs (including CT26-MFGE8KOEVs and MC38-MFGE8KOEVs) led to a significant decrease in αvβ3 expression and reduced co-localization of CRC-EVs with αvβ3 in macrophages compared to wild-type CRC-EVs (Fig. 5E and F). The gene discussed is DDX53; the disease is colorectal carcinoma.