To determine the type of cell death induced by circPIAS1 knockdown, sh-circPIAS1 HCC cells were treated with inhibitors of apoptosis (Z-VAD-FMK), necroptosis (necrostatin-1, Nec-1), and ferroptosis (ferrostatin-1, Fer-1, and liproxstatin-1, Lipro-1). This evidence concerns the gene PCSK1 and hepatocellular carcinoma.