The western Blot experiments confirmed that AICAR facilitated the phosphorylation of AMPK and the expression of GLUT3 in the mouse trophoblast cell membrane (Fig. 7C,D,G,H).In conclusion, AICAR up-regulated the expression of GLUT3 on the trophoblast cell membrane of GDM mice by activating AMPK. This evidence concerns the gene SLC2A3 and gestational diabetes.