PRF1 and neoplasm: The effects of the C5aR1hi macrophages pre-incubated with olaparib treated T127 tumor cells on granzyme B and perforin expression was reversed by C5aR1 inhibition during the co-culture of T127 from olaparib-treated mice and the C5aR1hi macrophages with no further induction of IFNγ compared to pre-incubation with olaparib-treated T127 tumor cells (Fig. 5B).