T127 tumor cells treated in vitro with AZD5305 and then cocultured in a transwell system with C5aR1 macrophages resulted in C5aR1 macrophages that suppressed T cell activation as assessed by positivity for GMZB, PRF1 or IFNγ (Supplementary Fig. 6K). The gene discussed is PRF1; the disease is neoplasm.