HSPG2 and hepatocellular carcinoma: Further validation analyses showed that among these 3 genes, IFRD1 was distinctly induced in CB-839-treated xenografts (Fig. 1d; Supplementary Figs. S1e, S2a, b), and consistently upregulated in response to glutamine deprivation in three various HCC cell lines (Fig. 1e, f) as well as CB-839-treated PLC/PRF/5 cells (Supplementary Fig. S2c, d).