Even though anti-PD-1 treatment decreased the percentage of cancer cells in subcluster “Autophagy” in DRG2-depleted tumors (Fig. 6G), this did not lead to inhibition of the tumor progression (Fig. 5C, D, F), possibly because of expansion of the subcluster “Spp1 + ” population. This evidence concerns the gene DRG2 and neoplasm.