Considering these findings and protein types, ROC curves were generated, especially focusing on CA1 and CA2, which are crucial hub proteins in disease progression, as found previously.3 These enzymes displayed higher abundance in PG compared to NP, in both western blot and mass spectrometry assays, and their AUC values approached 0.8, underscoring their potential as periodontitis progression biomarkers. This evidence concerns the gene CA1 and periodontitis.