AIF1 and infection: To quantify the loss of Iba-1 and TMEM119 immunoreactivity in the hippocampus, we used a nested ANOVA analysis, where one factor (individual sections in this example) was nested within another factor (infection/diagnosis), to explore whether the percent area covered by Iba-1 positive microglia differed in the gray matter (Fig. 7a) or in white matter (Fig. 7b) between groups.