Western blot results showed that the expression levels of IL-1β and TNF-α in the kidneys of DM mice were significantly increased compared with NC mice, and the expression levels of IL-1β and TNF-α in the kidneys of diabetic mice treated with 3-MA were significantly decreased compared with DM mice, which was in line with the results of ELISA (Figure 5C-E). Here, IL1B is linked to diabetes mellitus.