On the other hand, although PAK1KO had a high level of Treg (Fig. S7a, b), it also had an increase in PD1 + CD8 + T cell level (Fig. S7c, d) from one week to three weeks, suggesting that CD8 + T cells had increased anti-tumour activity in PAK1KO tumour over time. This evidence concerns the gene CD8A and neoplasm.