NOTCH3 and esophageal squamous cell carcinoma: Additionally, both mRNA and protein levels of Notch2 were significantly increased in ESCC tissues, serving as an independent predictor of poor OS and PFS.87 In vivo and in vitro experiments demonstrated that chemotherapy resistance in ESCC was associated with the down-regulation of Notch3 and simultaneous activation of EMT process.88 The ectopic expression of Notch3-activated forms inhibited EMT and increased sensitivity to chemotherapy, suggesting that Notch3 could be a potential biomarker for predicting favorable clinical outcomes in ESCC.