Regarding Notch4, researchers have verified that Notch4 expression is decreased in CRC, and the Notch4 mRNA level may serve as an independent prognostic factor for disease-free survival and overall survival (OS) in patients with CRC.61 Notch ligands, JAG1,62 JAG2,63 and DLL4,64 have also been reported to be significantly upregulated in CRC, and their high expression can predict a poor prognosis in CRC. This evidence concerns the gene JAG2 and colorectal carcinoma.