Additionally, highly expressed inducible nitric oxide synthase activates Notch1 through the TACE/ADAM17 pathway, promoting the CSC phenotype and enhancing HCC aggressiveness.393 These groundbreaking findings illuminate the role of the Notch signaling pathway in coordinating the self-renewal of liver CSCs, with potential implications for improving treatment strategies and limiting recurrence. The gene discussed is NOTCH1; the disease is hepatocellular carcinoma.