Firstly, heatmap analysis of the three differential genes including MYBL1, ARHGAP30 and FAM20A were performed and found that MYBL1 had the highest expression in early atherosclerotic tissues and the lowest expression in advanced atherosclerosis, while FAM20A and ARHGAP30 had the opposite trend (Fig. 2B). Here, FAM20A is linked to atherosclerosis.