To compare FFPE performance with other sample types and to assess the performance of the assay on IDH2 p.R140 detection, extracted DNA from the peripheral blood of three patients with hematologic malignancy (IDH1 p.R132H at VAF 46%, IDH2 p.140Q at 50% VAF, and wild-type) were tested at 200 ng, 100 ng, and 50 ng input in duplicate (Supplementary Table 4). The gene discussed is IDH1; the disease is hematologic disorder.