Intraperitoneal injection of the obtained G5-MTX nanoparticles to mice with ID8-VEGF ovarian tumors resulted in efficient depletion of TAMs, significant reduction of microvessel density, decrease in expression levels of VEGF-A, VEGF-C, mitigated resistance to anti-VEGF therapy, and improved survival. This evidence concerns the gene VEGFA and ovarian neoplasm.