Annexin V and propidium iodide (PI) apoptosis assays were used to study the ratio of cellular apoptosis in the cancer cell line H838 following treatment with OSTI−1716 and OSTI−2461 for 6 h since these analogues showed the greatest antiproliferative activity towards lung cancer cell line H838 among the tested cancer cell lines (Figure 9). This evidence concerns the gene ANXA5 and cancer.