HMGB1 and preeclampsia: In vitro studies have shown that heparinized adsorbents such as heparin sepharose deplete PF4, histones/nucleosomes, and HMGB1 [114].Heparin pre-treatment decreased serum and lung NETs in a C57BL/6J mice model [115].Circulating histones bound to H3 and H4 nucleosomes are increased in patients with preeclampsia and intrauterine growth restriction. H3 affects extravillous trophoblast migration, invasion, and survival. This effect can be reversed in vitro by LMWH, but not with ASA [69].