TNF and arthritic joint disease: In addition, 50, 100, and 400 mg/kg mangiferin inhibits mRNA expression of cytokine genes in the thymus and spleen of mice with induced-arthritis and lead to decreased serum levels of IL-1β, IL-6, TNF-α, and RANKL by downregulating NF-κB and activating ERK1/2 [355].