We exposed a monolayer of each cell type (HUVECs, and primary astrocyte and pericyte) to 1 MOI of icSARS-CoV-2-nMG and monitored the infection via fluorescent live cell imaging and measured productive infection through plaque assays using the supernatant from the mock and infected cells on VERO-TMPRSS2-ACE2 cells. The gene discussed is TMPRSS2; the disease is infection.