To check the interaction of PSPC1 with HCV viral RNA upon infection, HCV-JFH1 RNA was transfected into Huh7.5 cells for 48 h, followed by immunoprecipitation with anti-PSPC1 antibody along with anti-HuR antibody as a positive and IgG as a negative control, respectively. Here, ELAVL1 is linked to infection.