Although the CPSF6 staining did alter slightly, with an increase in smaller, more focused puncta in some instances, we did not see a significant change in the number or distribution of puncta following infection of Jurkat T-cells with HIV-1 VLPs, suggesting that HIV-1 CA is not driving CPSF6 relocalisation in this cell type. This evidence concerns the gene CPSF6 and infection.