By employing the strep-tagged HLA method and targeting specific HLA subtypes, we isolated predominant East Asian HLAs: HLA-A*11:01 and HLA-A*02:01 allele-restricted EBV epitopes from various NPC cell lines and validated their immunogenicity in EBV positive healthy donors, thereby expanding the EBV epitope database. Here, HLA-A is linked to nasopharyngeal carcinoma.