Secondly, it is important to note that Cx43 may have diverse roles at different stages of tumor development, while our study focused primarily on its role at the transcriptional level, neglecting other crucial levels such as post-transcriptional regulation, protein synthesis, and post-translational modifications, and we only partially validated our findings at the gene level, lacking proteomic validation that may not fully reflect the functional impact of Cx43 in KIRC biology; thus, further research is required to fully understand its role in KIRC. Here, GJA1 is linked to neoplasm.