All samples positive in the qPCR were negative in additional conventional PCR assays based on the 16S rRNA, dsb, gltA, sodB, omp-1, rpoB, groEL, and ftsZ genes and 23S-5S (ITS) intergenic region, mostly likely due the low bacteremia at the time of blood sampling. The gene discussed is HSPD1; the disease is bacterial infectious disease with sepsis.