The IgeneX Immunoblot revealed evidence of prior exposure to Lyme disease (IgG Immunoblot: 23 kda+ (Osp C), with weak positive bands at 31 kda (OspA) and 93 kda); Babesia titers and Babesia FISH were negative (B. microti, B. duncani), and a Bartonella Immunoblot IgM/IgG showed exposure to Bartonella species at the genus level, with positive exposure to B. quintana and negative Bartonella FISH. Here, CD40LG is linked to Lyme disease.