In addition, hierarchical clustering analysis of these DEPs shown in Figure 6C revealed the pathogenesis of acne was closely related to differential protein expression levels, including nuclear receptor-binding factor 2 (NRBF-2), cannabinoid receptor interacting protein 1a (CRIP1a) and acyl-CoA desaturase 1 (SCD-1), Phospholipase DDHD2 and Proteoglycan 4 (Prg4). The gene discussed is PRG4; the disease is acne.