To assess the effects of miR-99b-5p and Enz/Abi on EMT-mediated metastasis, the expression levels of EMT markers E-cadherin (epithelial), Snail (EMT-TF), N-cadherin and Vimentin (mesenchymal), and Snail were examined using immunofluorescence in EA PCa (LNCaP, 22Rv1, and C4-2B) and AA PCa (MDA PCa 2b) cells transfected with nonsense RNA or miR-99b-5p mimic in the absence/presence of Enz or Abi. The gene discussed is TF; the disease is Esophageal atresia.