To investigate the contribution of TLRs to the enhanced anti-tumor activity of macrophages in HK-C60 administration, we established a melanoma model with HK-C60 administration in WT, TLR2 or TLR4-KO mice following the protocol represented in Figure 1A. Interestingly, either TLR2-KO or TLR4-KO mice showed progressed tumor growth compared to WT mice in HK-C60 administered condition (Figure 5I). The gene discussed is TLR2; the disease is melanoma.