Moreover, the 3D scaffold was divided into two areas, an inner that was surface functionalized with fibronectin (FN) and a surrounding that was coated with collagen (COL) type I. The two distinct areas were developed in order to create optimal growth conditions for the PANC-1 cancer cells (inner area) and the stromal compartments consisting of the PS-1 stellate and HMECs endothelial cells (surrounding area). This evidence concerns the gene FN1 and cancer.