Aloperine exerted its antitumor activity through the promotion of caspase-dependent apoptosis (and not regulating the cell cycle as shown in colon cancer cell line [57]), as determined by flow cytometry (FC) using the Annexin V-FITC/PI staining assay, and by western blot analysis (WB) examining the expression of PARP, Bcl-xL, Bid, caspase-3, -8, and -9 in the cells incubated with aloperine [21]. The gene discussed is BID; the disease is malignant colon neoplasm.