In the immortalized OvCa3 A hTERT cells, Sox2 (RQ 0.130 ± 0.070), p21 (RQ 0.270 ± 0.001), p53 (RQ 0.830 ± 0.064), and c-myc (RQ 0.869 ± 0.001) expression was downregulated compared to the primary ovarian cancer cells OvCa3 A. Only Oct4 (RQ 3.263 ± 0.051) was overexpressed in the OvCa3 A hTERT cells (Figure 4a). The gene discussed is POU5F1; the disease is ovarian cancer.