In the immortalized OvCa7 A hTERT cells, Sox2 (RQ 3.819 ± 0.189) and p53 (RQ 1.466 ± 0.110) expression was upregulated compared to the primary ovarian cancer cells OvCa7 A. In contrast, the expression of Oct4 (RQ 0.188 ± 0.051), c-myc (RQ 0.649 ± 0.092), and p21 (RQ 0.019 ± 0.004) in the immortalized OvCa7 A hTERT cells was downregulated (Figure 4a). The gene discussed is POU5F1; the disease is ovarian cancer.