MTOR and colorectal carcinoma: Indeed, although it was verified, using three distinct CRC cell lines (RKO, SW480, and HCT116 cell lines), that the combination of MET + ASP has a striking additive effect on AMPK (5′adenosine monophosphate-activated protein kinase) activation and mTOR (mammalian target of rapamycin) inhibition, with increased autophagy [30], and that this combination showed an additive/synergic effect in tumor spheroids generated in suspension from patient-derived CRCs, there was no additive effect when these two compounds were combined in 2D cultures of the CRC HCT-15 cell line [31].