Breast cancers are highly complicated and a variety of therapeutic strategies have attempted to treat them, e.g., therapeutic alteration of pathway components, applications of immune checkpoints, androgen inhibitors [31], etc. Here, we study the role of Vitamin D3-gold nanoparticle treatment in the alteration of the Hippo pathway in ER (+) (MCF-7 cells) and TNBC (MDA-MB-231 cells). Here, ESR1 is linked to breast carcinoma.