Having confirmed the functionality and specificity of our CD301 probes, recombinant CD301 multimers were applied for the staining of paraffin-embedded osteosarcoma specimens (n = 50) with normal corresponding tissue (n = 2), as well as 12 different normal human tissues, all arranged on tissue microarrays (Figure 1C–J; Supplementary Figures S3 and S5). Here, CLEC10A is linked to osteosarcoma.