RB1 and non-small cell lung carcinoma: To date, it has been demonstrated in primary and immortalized cultures (human dermal fibroblasts; alveolar epithelial cells; non-small-cell lung cancer—A549 cells; and primary and telomerase immortalized human corneal limbal epithelial cells) that MmC-treated cells display characteristics of senescent cells, such as abrogated proliferation, a stable change in cell morphology, intensified senescence-associated β-galactosidase (SA-β-gal) activity, and a transient increase in the ROS level, upregulated SASP factors, p21, p53, retinoblastoma (Rb) genes, etc. [27,28,29,30].