In the following sections, we will detail specific pathways activated by muscarinic signaling that allow for cellular proliferation, such as Wnt/Apc/β-catenin, MAPK/ERK, epidermal growth factor receptor (EGFR), and, for cellular migration, E-cadherin, matrix metalloproteinase (MMP), osteopontin, PI3K/AKT in gastric, pancreatic, and colon cancers. This evidence concerns the gene EGFR and colonic neoplasm.