To elucidate if Sox10 expression is indeed regulated by Tle4 and Hes1, we used S16 Schwannoma cells and quantified the signal intensity of endogenous Sox10 immunofluorescence in these cells by flow cytometry after transfecting them with control plasmids or plasmids encoding Hes1, Tle4, or both. Here, HES1 is linked to schwannoma.