We customized our EV-CATCHER assay with an anti-PLAP antibody and then used our optimized small-RNA cDNA library preparation protocol to conduct NGS analyses [66] where we compared the miRNA profiles of circulating placental EVs purified from the plasma of pregnancies with placenta percreta (cases) with those purified from plasma of placenta previa pregnancies (controls). The gene discussed is ALPP; the disease is placenta praevia.