Of the five T-cell clones with specific reactivity against cell lines transduced with DNMT3AR882H, one T-cell clone was able to recognize and lyse HLA-A*01:01-positive DNMT3AR882H patient-derived AML cells, demonstrating that YTDVSNMSHLA is a neoantigen in AML. Here, HLA-A is linked to acute myeloid leukemia.