Despite it not being detected with mass spectrometry, we successfully isolated a T-cell clone that specifically reacts to HLA-A*01:01- and DNMT3AR882H-positive patient-derived AML samples, thereby confirming that YTDVSNMSHLA is an HLA-A*01:01-binding neoantigen. The gene discussed is HLA-A; the disease is acute myeloid leukemia.