E2 treatment significantly increased the protein expression of PPAR-γ2 in differentiated lipedema adipocytes both in 2D monolayer culture (1.5-fold, Figure 5B, Supplementary Figure S4) and spheroids (3-fold, Figure 6B, Supplementary Figure S5) compared to untreated control lipedema (Figure 5 and Figure 6). This evidence concerns the gene PPARG and Lipedema.