To further understand the extent of neuronal damage in response to ALS-CSF, as well as the effectiveness of IMS-088 in ameliorating neuronal pathology, we examined the distribution by immunofluorescence microscopy and expression levels of neurofilament NfL, NfM, and NfH proteins by immunoblotting of spinal cord extracts. This evidence concerns the gene NEFH and amyotrophic lateral sclerosis.