Using antibody against phospho-TDP-43 (Cosmobio P01), immunoblotting of spinal cord extracts revealed that ALS-CSF infusion in hTDP-43WT mice increased the levels of phosphorylated TDP-43 species (human and mouse) recovered in the soluble and insoluble fractions (Figure 3D,E). Here, TARDBP is linked to amyotrophic lateral sclerosis.