NGS analysis was performed using the Nephropathy Solution (NES) kit (Sophia Genetics, Lausanne, Switzerland) covering the coding regions of 44 most clinically relevant genes related to a broad range of nephropathies (AGXT, AQP2, ATP6V0A4, ATP6V1B1, AVPR2, BSND, CASR, CEP290, CLCN5, CLCNKB, COL4A3, COL4A4, COL4A5, CRB2, CTNS, CUBN, CYP24A1, DSTYK, EMP2, EYA1, FN1, FOXC1, GRHPR, HNF1b, KANK2, KCNJ1, LAMB2, NPHS2, NR3C2, OCRL, PAX2, PHEX, PKD1, PKD2, PKHD1, SIX1, SLC12A1, SLC12A3, SLC34A1, SLC4A1, SLC4A4, TTC21B, UMOD, WT1). The gene discussed is COL4A5; the disease is kidney disorder.