However, we identified one sample from a patient with HCL that was BRAF V600 E/E2/D-positive by the Idylla BRAF assay, which harbored BRAF c.1799T > A (p.Val600Glu) and BRAF c.1824T > G (p.His608Gln) variants in cis at VAFs of 14%, as identified by NGS (Figure 4). Here, BRAF is linked to hairy cell leukemia.