To further confirm the accumulation of neutrophils in CRC, we digested tissues from all 39 samples into single‐cell suspension using lysis buffers, and then stained cells with classical markers of myeloid cells (CD14, CD11b, CD15, CD66b, CD33, and HLA‐DR) and examined them by flow cytometry. This evidence concerns the gene ITGAM and colorectal carcinoma.