To confirm existence of autocrine loop via secretion of LIF into the medium by OCa cells, we plated several established cell lines (OV90, TOV112D, TOV21G, IGROV1, ES2, OVSAHO, COV644) and primary OCa cell lines (OCa30, OCa39, OCa50), and let them grow to 70-80% confluency. The gene discussed is LIF; the disease is oculocutaneous albinism.