In proof-of-concept experiments, SSEA-4 specific CAR T cells specifically interacted with cells from the SSEA-4 positive teratocarcinoma cell line NTERA-2 (Fig. 5B), resulting in efficient cytolysis of tumor cells (Fig. 5C), target-induced degranulation responses by CD107a upregulation (Fig. 5D) and secretion of IL-2, TNF-α and IFN-γ in a strictly antigen-specific manner (Fig. 5E). Here, TNF is linked to teratocarcinoma.