Two lentiviral vectors encoding efficient shRNAs targeting Nur77 were used to knockdown endogenous Nur77 expression in the three cell lines with high Nur77 expression (Fig. 2A and Fig. S1I), as it was previously noted that Nur77 was variably expressed in the panel of ESCC cell lines. This evidence concerns the gene NR4A1 and esophageal squamous cell carcinoma.