Immunohistochemistry analysis showed that TNF-α, IL-6 and IL-10 were mainly expressed by mesangial cells and infiltrating macrophages in the glomeruli, as well as in the tubulointerstitial area and endothelium during the course of tubulointerstitial and glomerular nephritis in ECM [19,28]. This evidence concerns the gene TNF and glomerulonephritis.