In this study, we aimed to (i) detect Td-dentilisin and MMP-8 immunoexpression levels in gingival tissue samples of patients with periodontitis compared with periodontally healthy gingivae to (ii) assess the MMP-8, MMP-2, MMP-7, TIMP-1, and IL-1β mRNA expressions in the diseased vs. healthy gingiva. Here, TIMP1 is linked to periodontitis.